ifn β promoter luciferase reporter (Addgene inc)
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ifn β promoter luciferase reporter
Ifn β Promoter Luciferase Reporter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ifn+%CE%B2+promoter+luciferase+reporter/IFN-Beta_pGL3+(Plasmid+%23102597)/pm39404379-79-14-19
Average 94 stars, based on 62 article reviews
Ifn β Promoter Luciferase Reporter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ifn+%CE%B2+promoter+luciferase+reporter/IFN-Beta_pGL3+(Plasmid+%23102597)/pm39404379-79-14-19
Average 94 stars, based on 62 article reviews
ifn β promoter luciferase reporter - by Bioz Stars,
2026-09
94/100 stars
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Luciferase:Article Title: A Gain-of-Function Cleavage of TonEBP by Coronavirus NSP5 to Suppress IFN-β Expression Article Snippet: Fluorescent blot detection was performed using an Odyssey ® CLx Imaging System (LI-COR). .. HEK293T cells were co-transfected with the indicated luciferase constructs of TonE-driven Photinus [ ], or Article Title: A Gain-of-Function Cleavage of TonEBP by Coronavirus NSP5 to Suppress IFN-β Expression. Article Snippet: Fluorescent blot detection was performed using an Odyssey® CLx Imaging System (LI-COR). .. HEK293T cells were co-transfected with the indicated luciferase constructs of TonEdriven Photinus [15], or Article Title: Identification of a Papain-Like Protease Inhibitor with Potential for Repurposing in Combination with an Mpro Protease Inhibitor for Treatment of SARS-CoV-2 Article Snippet: .. An Addgene plasmid was used for the Article Title: Identification of a Papain-Like Protease Inhibitor with Potential for Repurposing in Combination with an M pro Protease Inhibitor for Treatment of SARS-CoV-2 Article Snippet: .. An Addgene plasmid was used for the Article Title: A Gain-of-Function Cleavage of TonEBP by Coronavirus NSP5 to Suppress IFN-β Expression Article Snippet: The plasmid encoding the N-terminal fragment of TonEBP generated by SARS-CoV-2 NSP5 cleavage was cloned into the pCMV-tag 2B vector (#211172, Agilent Technologies, Santa Clara, CA, USA) by amplification and standard restriction cloning. .. The Article Title: A Gain-of-Function Cleavage of TonEBP by Coronavirus NSP5 to Suppress IFN-β Expression. Article Snippet: The plasmid encoding the N-terminal fragment of TonEBP generated by SARS-CoV-2 NSP5 cleavage was cloned into the pCMV-tag 2B vector (#211172, Agilent Technologies, Santa Clara, CA, USA) by amplification and standard restriction cloning. .. The Construct:Article Title: A Gain-of-Function Cleavage of TonEBP by Coronavirus NSP5 to Suppress IFN-β Expression Article Snippet: Fluorescent blot detection was performed using an Odyssey ® CLx Imaging System (LI-COR). .. HEK293T cells were co-transfected with the indicated luciferase constructs of TonE-driven Photinus [ ], or Article Title: A Gain-of-Function Cleavage of TonEBP by Coronavirus NSP5 to Suppress IFN-β Expression. Article Snippet: Fluorescent blot detection was performed using an Odyssey® CLx Imaging System (LI-COR). .. HEK293T cells were co-transfected with the indicated luciferase constructs of TonEdriven Photinus [15], or Plasmid Preparation:Article Title: A Gain-of-Function Cleavage of TonEBP by Coronavirus NSP5 to Suppress IFN-β Expression Article Snippet: Fluorescent blot detection was performed using an Odyssey ® CLx Imaging System (LI-COR). .. HEK293T cells were co-transfected with the indicated luciferase constructs of TonE-driven Photinus [ ], or Article Title: A Gain-of-Function Cleavage of TonEBP by Coronavirus NSP5 to Suppress IFN-β Expression. Article Snippet: Fluorescent blot detection was performed using an Odyssey® CLx Imaging System (LI-COR). .. HEK293T cells were co-transfected with the indicated luciferase constructs of TonEdriven Photinus [15], or Article Title: Identification of a Papain-Like Protease Inhibitor with Potential for Repurposing in Combination with an Mpro Protease Inhibitor for Treatment of SARS-CoV-2 Article Snippet: .. An Addgene plasmid was used for the Article Title: Identification of a Papain-Like Protease Inhibitor with Potential for Repurposing in Combination with an M pro Protease Inhibitor for Treatment of SARS-CoV-2 Article Snippet: .. An Addgene plasmid was used for the Control:Article Title: A Gain-of-Function Cleavage of TonEBP by Coronavirus NSP5 to Suppress IFN-β Expression Article Snippet: Fluorescent blot detection was performed using an Odyssey ® CLx Imaging System (LI-COR). .. HEK293T cells were co-transfected with the indicated luciferase constructs of TonE-driven Photinus [ ], or Article Title: A Gain-of-Function Cleavage of TonEBP by Coronavirus NSP5 to Suppress IFN-β Expression. Article Snippet: Fluorescent blot detection was performed using an Odyssey® CLx Imaging System (LI-COR). .. HEK293T cells were co-transfected with the indicated luciferase constructs of TonEdriven Photinus [15], or Transfection:Article Title: A Gain-of-Function Cleavage of TonEBP by Coronavirus NSP5 to Suppress IFN-β Expression Article Snippet: Fluorescent blot detection was performed using an Odyssey ® CLx Imaging System (LI-COR). .. HEK293T cells were co-transfected with the indicated luciferase constructs of TonE-driven Photinus [ ], or Article Title: A Gain-of-Function Cleavage of TonEBP by Coronavirus NSP5 to Suppress IFN-β Expression. Article Snippet: Fluorescent blot detection was performed using an Odyssey® CLx Imaging System (LI-COR). .. HEK293T cells were co-transfected with the indicated luciferase constructs of TonEdriven Photinus [15], or Expressing:Article Title: A Gain-of-Function Cleavage of TonEBP by Coronavirus NSP5 to Suppress IFN-β Expression Article Snippet: The plasmid encoding the N-terminal fragment of TonEBP generated by SARS-CoV-2 NSP5 cleavage was cloned into the pCMV-tag 2B vector (#211172, Agilent Technologies, Santa Clara, CA, USA) by amplification and standard restriction cloning. .. The Article Title: A Gain-of-Function Cleavage of TonEBP by Coronavirus NSP5 to Suppress IFN-β Expression. Article Snippet: The plasmid encoding the N-terminal fragment of TonEBP generated by SARS-CoV-2 NSP5 cleavage was cloned into the pCMV-tag 2B vector (#211172, Agilent Technologies, Santa Clara, CA, USA) by amplification and standard restriction cloning. .. The |
![The N-terminal sequence of Nsp1 is not specific for translation inhibition. (A) The colocalization of EGFP and EGFP-fused Nsp1/126–180 (green) with ribosome (immunostained with anti-RPS6 antibody [red]) was analyzed by immunofluorescence in HEK293T cells. The intensity profile describes the distribution of EGFP or EGFP-fused Nsp1/126–180 and RPS6-specific fluorescence along the indicated line. (B) Pearson’s correlation between Nsp1 or its truncated mutants and RPS6 was analyzed and displayed. The P value was calculated in GraphPad Prism 8.2.1 using ordinary one-way ANOVA and subjected to multiple-testing correction by FDR method. (C to F) HEK293T cells (5 × 10 4 cells/well, 48-well plates) were cotransfected with 50 ng firefly luciferase reporter plasmid for IFN-β promoter (IFNβ-Luc), 5 ng Renilla luciferase reporter plasmid for the herpes simplex virus thymidine kinase promoter (HSV-TK-Luc; used as an internal control), and 200 ng of the indicated truncated plasmid or EGFP fusion-expressing plasmid. SeV infection was performed at 24 h posttransfection. The reporter activity was detected by dual-luciferase reporter system at 10 h postinfection. Data in panels C to F are means and SEM from at least three independent experiments and were normalized to the control group.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_2758/pmc08552758/pmc08552758__spectrum.00169-21-f004.jpg)